The department owns a Sable Systems/Li-Cor differential respirometry setup which can be used to measure carbon dioxide, oxygen, water vapour and activity. CO2 is measured with a Li-7000 CO2 analyser (LiCor, Lincoln, NB, USA) and O2 with an Oxzilla FC-2 O2 analyser (Sable systems, Las Vegas, NV, USA).
We can modify temperature between -10 and +50°C and have successfully measured metabolic processes related to basal metabolism, diapause, starvation, growth and active movement. Many kinds of critters have been used, from Drosophilid flies, beetles, butterflies even to human blood.
We are always interested in collaborations. While the setup is optimized for measuring insects and small animals (< 1 g) the setup is very flexible and can be used to accommodate different (air-based) respirometry needs, including the measurement of vertebrates.

For more information, contact: Philipp Lehmann

Examples of studies where different respirometry-methods have been used

  • Woronik A, Constanti S, Käkelä R, Wheat C & Lehmann P. Physiological differences between female limited, alternative reproductive strategies: the Alba phenotype in the butterfly Colias croceus. In review.
  • Emami SN, Lindberg BG, Hua S, Hill S, Mozuraitis R, Lehmann P, Birgersson G, Borg-Karlson AK, Ignell R & Faye I (2017) A key malaria metabolite modulates vector blood seeking, feeding and susceptibility to infection. Science, 355, 1076-1080.
  • Kivelä S, Lehmann P & Gotthard K (2016) Does oxygen supply constrain metabolism of larval insects before moulting: an empirical test at the individual level. Journal of Experimental Biology, 219, 3061-3071.
  • Lehmann P, Pruisscher P, Posledovich D, Carlsson M, Käkelä R, Tang P, Nylin S, Wheat CW, Wiklund C & Gotthard K (2016) Energy and lipid metabolism during direct and diapause development in a pierid butterfly. Journal of Experimental Biology, 219, 3049-3060.
  • Stålhandske S, Lehmann P, Pruisscher P & Leimar O (2015) Effect of winter cold duration on spring phenology of the orange tip butterfly, Anthocharis cardamines. Ecology & Evolution, 5, 5509-5520